Description
Principle of the Assay: MMP28 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP28 antibody and an MMP28-HRP conjugate. The assay sample and buffer are incubated together with MMP28-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP28 concentration since MMP28 from samples and MMP28-HRP conjugate compete for the anti-MMP28 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP28 from the sample, fewer sites are left to bind MMP28-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP28 concentration in each sample is interpolated from this standard curve. 3. MATERIALS All reagents provided are stored at 2-8 degree C. Refer to the expiration date on the label. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES